种质
生物
生殖系发育
体细胞
生殖细胞
细胞生物学
外胚层
遗传学
胚状体
细胞命运测定
基因
胚胎干细胞
胚胎
原肠化
诱导多能干细胞
转录因子
作者
Mitinori Saitou,Sheila C. Barton,M. Azim Surani
出处
期刊:Nature
[Springer Nature]
日期:2002-07-01
卷期号:418 (6895): 293-300
被引量:791
摘要
Germ cell fate in mice is induced in proximal epiblast cells by the extra-embryonic ectoderm, and is not acquired through the inheritance of any preformed germ plasm. To determine precisely how germ cells are specified, we performed a genetic screen between single nascent germ cells and their somatic neighbours that share common ancestry. Here we show that fragilis, an interferon-inducible transmembrane protein, marks the onset of germ cell competence, and we propose that through homotypic association, it demarcates germ cells from somatic neighbours. Using single-cell gene expression profiles, we also show that only those cells with the highest expression of fragilis subsequently express stella, a gene that we detected exclusively in lineage-restricted germ cells. The stella positive nascent germ cells exhibit repression of homeobox genes, which may explain their escape from a somatic cell fate and the retention of pluripotency.
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