细胞外基质
汤剂
医学
纤维化
基质金属蛋白酶
肾
类黄酮
下调和上调
糖尿病
药理学
内科学
化学
内分泌学
抗氧化剂
生物化学
基因
作者
Chengshan JIN,Xiaotian WU,Yue YOU,Yuing WANG,Jing Wu,Along ZUO,Yan Zheng,Jianpeng GUO
标识
DOI:10.1016/s1875-5364(23)60434-0
摘要
Diabetic kidney disease (DKD) is the most common complication of diabetes mellitus (DM). Qianjin Wenwu decoction (QWD), a well-known traditional Korean medicine, has been used for the treatment of DKD, with satisfactory therapeutic effects. This study was designed to investigate the active components and mechanisms of action of QWD in the treatment of DKD. The results demonstrated that a total of 13 active components in five types were found in QWD, including flavonoids, flavonoid glycosides, phenylpropionic acids, saponins, coumarins, and lignins. Two key proteins, TGF-β1 and TIMP-1, were identified as the target proteins through molecular docking. Furthermore, QWD significantly suppressed Scr and BUN levels which increased after unilateral ureteral obstruction (UUO). Hematoxylin & eosin (H&E) and Masson staining results demonstrated that QWD significantly alleviated renal interstitial fibrosis in UUO mice. We also found that QWD promoted ECM degradation by regulating MMP-9/TIMP-1 homeostasis to improve renal tubulointerstitial fibrosis and interfere with the expression and activity of TGF- β1 in DKD treatment. These findings explain the underlying mechanism of QWD for the treatment of DKD, and also provide methodological reference for investigating the mechanism of traditional medicine in the treatment of DKD.
科研通智能强力驱动
Strongly Powered by AbleSci AI