纳米花
生物分子
生物分析
检出限
生物传感器
带隙
拉曼散射
基质(水族馆)
化学
纳米技术
分析化学(期刊)
材料科学
色谱法
光电子学
拉曼光谱
纳米结构
物理
海洋学
地质学
光学
作者
Lingling Jiang,Jiale Zhang,Hongying Li,Chaoqin He,Ruo Yuan,Yingqi Zhao,Jian‐An Huang,Ying Zhuo,Xia Yang
标识
DOI:10.1016/j.cej.2023.142102
摘要
Metal oxide-based surface-enhanced Raman scattering (SERS) platforms have attracted considerable interest, which show potentials for biomolecule detection due to their wide bandgap tuning range, stable properties, high electron mobility and good optical properties. However, the types and enhancement factor (EF) of metal oxide SERS substrates are limited. And the band-matched efficient photo-induced charge transfer (PICT) is the important process to further improve the EF. In this study, one efficient SERS substrate Sn1-xCexO2 was synthesized by varying concentrations of Ce ions with band gap tuning and oxygen vacancies introducing. As a result, the charge transfer pathway and strong interaction between the Sn1-xCexO2 nanoflowers (NFs) and methylene blue (MB) molecules are promoted, and the enhancement factor (EF) of this Sn1-xCexO2 (x = 0.03) NF is as high as 1.80 × 106. Then, we constructed a biosensor based on hybridization chain reaction (HCR) strategy combining with Mg2+-dependent DNAzyme cleavage nucleic acid cascade signal amplification to achieve sensitive and quantitative detection of microRNA 21 (miRNA 21). The limit of detection (LOD) was 0.72 fM with a detection linearity ranged from 1 fM to 10 nM. This study gives an approach for preparation of new SERS substrates which can be used in bioanalysis.
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