Effects of plant growth regulators on ultrastructure of soybean seed

In this study,Dongnong 46 was chosen as the experimental material.During its growth period,four kinds of plant growth regulators(TIBA,RE,SBS and DTA- 6) were used to spray the leaves of Dongnong 46.Through the observation of the submicroscopic structure of the soybean storage cells,it could be found that at R6stage,TIBA took a part in the proteome aggregating process.Under the SBS treatment,the proteome were turned to out of shape and there were more starch grains in the storage cells.RE and DTA-6 increased the number of proteome and lipid bodies obviously.At R7stage,the effects of TIBA on the aggregation of proteome were not evident,while it increased the number of lipid bodies and made them arranged more closely.In the samples with RE treatment,there were still more proteome and lipid bodies in the storage cells.In the SBS treated samples,there were more starch grains and proteome.The number of proteome in the storage cells was increased and their shape and size were heterogeneity after DTA-6 treated,the average diameters of proteome were larger than the control groups.At R8stage,the substances were rich in the storage cells and the sizes of proteome were larger.In the RE,SBS and DTA-6 treated samples,more proteome existed in the storage cells.In RE groups,the proteome were arranged more closely.In DTA-6 groups,the size of proteome increased,however,there were still unconverted starch grains in the storage cells,indicating that DTA-6 had slowed the degradation and conversion speed of the starch grains.In SBS treated groups,the lipid bodies were arranged loosely in the storage cells,but in RE and TIBA groups,the lipid bodies were full and arranged closely.This experiment showed that the plant growth regulators had a significant effect on the growth on soybean storage cells.In some certain stages of soybean growth,the purpose of increasing production and improving the quality could be achieved through spraying the four selected regulators.More specifically,in RE and DTA-6 treated samples,the production can be increased by 17% and 21.83%,respectively.The plants spraying with TIBA showed obvious influence on plant dwarf and stem strong,the height of the plants decreased from 28% to 30%,the capability of lodging-resistance were improved significantly.