互补DNA
分子生物学
底漆(化妆品)
基因亚型
生物
cDNA文库
分子质量
过敏原
表位
肽序列
氨基酸
化学
生物化学
遗传学
基因
抗原
酶
免疫学
过敏
有机化学
作者
Jean‐Marc Chatel,Hervé Bernard,Frank M. Orson
出处
期刊:International Archives of Allergy and Immunology
[S. Karger AG]
日期:2003-01-01
卷期号:131 (1): 14-18
被引量:86
摘要
Ara h 2 is a major peanut allergen recognized by IgE in more than 90% of patients. After electrophoretic separation the purified protein exists as a doublet, and sequences of one incomplete cDNA and one genomic clone for this allergen have been reported.Ara h 2 isoforms were purified and analyzed by mass spectroscopy, and PCR amplification products of Ara h 2 were cloned and sequenced.Mass spectroscopy of purified Ara h 2 clearly identified a molecular doublet of 16,670 and 18,050 Daltons. Amplification of a peanut cDNA library using PCR primer pairs located at the amino- and carboxy-terminus revealed 2 bands separated by 50 base pairs, which we cloned and sequenced. Two types of complete cDNA clones were obtained, Ara h 2.01 and Ara h 2.02. Compared to Ara h 2.01 and the previously reported cDNA sequences, Ara h 2.02 is characterized by a 12 amino acid insertion starting at position 75 that contains a third repeat of the major IgE binding epitope DPYSPS.We demonstrated the molecular and genetic characteristics of two Ara h 2 isoforms, revealing that one, Ara h 2.02, might be the more potent allergen.
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