Effects of α-Anordrin on the Growth of Androgen-Dependent Human Prostate Cancer Cell Line LNCaP

Objective To investigate the effects of α-anordrin(α-ANO)on the growth of androgendependent human prostate cancer cell line LNCaP.Methods The monomers of α-ANO and β-ANO were purified through alumina column chromatographic separation.SRB staining method was used to detect the effects of α-ANO on the growth of LNCaP cells and on antagonizing R1881(a kind of androgen stimulant) in inducing LNCaP cells proliferation.Cell morphology was examined under microscope.ELISA was used to examine the effect of α-ANO on PSA secretion by LNCaP cells.Results In normal medium,after LNCaP cells were treated with α-ANO at 8,12,16,24 and 32 μmol/L,cell survival rates were 85.8%,51.5%,40.8%,29.2%,1.0% respectively.In the androgen-depleted medium,α-ANO could antagonize LNCaP cell proliferation induced by R1881.The PSA secretion of LNCaP cells was also decreased.Conclusion α-ANO inhibits the proliferation of androgen-dependent human prostate cancer cell line LNCaP.In the presence of androgen,α-ANO antagonizes the LNCaP cell proliferation induced by androgen.