Enzyme activities distributions of different treatments in the soil profile in which alfalfa and winter wheat were planted continuously and the fluctuations of enzyme activities of different fertilizers treatments were studied. The results showed that the enzyme activities reduced from the soil surface to the deep soil layers, but the enzyme activities distributions of different treatments were not significantly different. When the winter wheat was growing, the catalase activities of different treatments were increasing and the fluctuations of alkaline phosphatase and available P were correlated tach other.