Analysis of the results of antinuclear antibody spectrum for 5971 cases which antinuclear antibody were negative

Objective To explore the value of the combined of antinuclear and antibodies(ANA) and antinuclear antibody spectrum(ANAs) in autoimmune disease(AID).Methods A total of 5971 Chinese patients with antinuclear antibodies negative were studied.All of them were detected the ANAs by Europe-Mongolia immunity print,while ANA was measured by Europe-Mongolia indirect immunoflurescence assay.Results There were 422 cases which ANA were negative showed positive in ANAs(7.07%).The positive rate of anti-SSA,anti-SSB,anti-ScL-70,anti-PM-Scl,anti-Jo-1,anti-PCNA,anti-dsDNA,anti-AHA,anti-ARPA,anti-AMA-M2was1.46%,0.33%,0.35%.0.35%,0.28%,0.32%,0.83%,0.63%,0.60% and 0.75% respectively.The positive rate of anti-Ro-52 was the highest(2.04%),and the second one was anti-SSA(1.46%).The frequency of anti-PCNA was associated with patients'age(χ 2 = 6.33,P 0.05).The positive rate of only one antibody positive in ANAs was statistically higher in female than in male(χ 2 = 6.51,P 0.05).Conclusion The results in this study suggest screening with both ANA and ANAs can improve the diagnosis of AID.