Effects of bone marrow mesenchymal stem cells cocultured with pancreatic cells on hyperglycemia in streptozotocin-induced diabetic rats

Objective To observe if the bone marrow mesenchymal stem cells(BMSCs)cocultured with pancreatic cells can be transformed into islet-like cells,and clarify the effect of blood glucose control of BMSCs in diabetic rats.Methods BMSCs and pancreatic cells were concultured in vitro.18SD rats were introperitonally injected with streptozotocin (STZ)to set up animal models of diabetes and the rat models were randomly divided into PBS group,BMSCs group and BMSCs cocultured with pancreatic cells group (n=6).The bromodeoxyuridine (Brdu)-labeled cells were transplanted into STZ-inducd diabetic rats by caudal vein,the clinical efficacy was observed.The distrubition of Brdu-labeled cells in the pancreas was examined by immunohistochemistry. Results The blood glucose in caudal vein was detected by blood glucose detection instrument.The blood glucose level in PBS group didn’t change compared with before transplantation (P0.05).The blood glucose level was reduced from the 14th day after transplantation in BMSCs group and BMSCs cocultured with pancreatic cells group, there were significant differences compared with before transplantation(P0.01).There was significantdifferenceof the blood glucose level after transplantation between BMSCs group and BMSCs cocultured with pancreatic cells group (P0.01).The immunohistochemical results showed that the Brdu-labeled cells with positive insulin-expression were tested in the BMSCs group and BMSCs cocultured with pancreatic cells group,but they were not found in PBS group.Conclusion The cultured cells could reduce hyperglycemia in the STZ-induced diabetic rats by caudal vein.BMSCs could also reduce hyperglycemia in the STZ-induced diabetic rats by caudal vein.The effect of the later was better than the former.