Preparation of Calcium Alginate Microspheres used for Drug Carrier by Emulsification-gelation Method

Calcium-Alginate microspheres were prepared by emulsification-gelation.The impact of experimental condition such as the concentration of sodium alginate,calcium chloride concentration,emulsifying speed,the relative proportion of organic phase to aqueous phase and emulsifier concentration to particle size and morphology were investigated.The drug loading profile of the microspheres was studied by using hemoglobin as a model drug.Microspheres with ideal morphology could be obtained under the optimized condition.The diameter of the microspheres was about 1μm.Drug-loading rate is relatively low.The drug-loading rate increases with increase of hemoglobin concentration,but when the bovine hemoglobin concentration is above 10 mg/mLt,he drug loading rates increase not obviously.