Optimization and Application of CRISPR/Cas-based in planta Gene Targeting Technologies

CRISPR clustered regularly interspaced short palindromic repeats crRNA CRISPR-RNA D-loop displacement-loop DSB Double-strand break dsDNA double-stranded DNA gDNA genomic DNA gRNA guide RNA GT Gene Targeting HR Homologous Recombination ipGT in planta GT MMEJ Microhomology-Mediated end-joining NHEJ Non-homologous end joining PAM Protospacer Adjacent Motif pre-crRNA precursor crRNA scFv single-chain variable fragment sfGFP superfolder-Green Fluorescent Protein sgRNA single guide RNA SSA single strand annealing ssDNA single-stranded DNA SunTag SuperNova tagging tracrRNA trans-activating crRNA ttLbCas12a-i intron-containing temperature-tolerant LbCas12a