DNA折纸
纳米技术
背景(考古学)
DNA纳米技术
DNA
纳米结构
表征(材料科学)
材料科学
计算机科学
生物
遗传学
古生物学
作者
Grant A. Knappe,Eike‐Christian Wamhoff,Mark Bathe
标识
DOI:10.1038/s41578-022-00517-x
摘要
DNA origami has emerged as a powerful method to generate DNA nanostructures with dynamic properties and nanoscale control. These nanostructures enable complex biophysical studies and the fabrication of next-generation therapeutic devices. For these applications, DNA origami typically needs to be functionalized with bioactive ligands and biomacromolecular cargos. Here, we review methods developed to functionalize, purify and characterize DNA origami nanostructures. We identify remaining challenges such as limitations in functionalization efficiency and characterization. We then discuss where researchers can contribute to further advance the fabrication of functionalized DNA origami. DNA origami nanostructures are useful constructs for biophysical and therapeutic studies. This Review discusses how these nanostructures are functionalized with bioactive conjugates, purified and characterized, and compares the advantages and limitations of these methods in the context of different applications.
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