三氯生
微生物学
四环素
氯霉素
苯扎溴铵
环丙沙星
大肠杆菌
化学
交叉电阻
抗生素耐药性
抗生素
多重耐药
链霉素
生物
医学
生物化学
有机化学
病理
基因
作者
Jiratchaya Puangseree,Saharuetai Jeamsripong,Rangsiya Prathan,Chanika Pungpian,Rungtip Chuanchuen
出处
期刊:Food Control
[Elsevier]
日期:2021-06-01
卷期号:124: 107892-107892
被引量:20
标识
DOI:10.1016/j.foodcont.2021.107892
摘要
A total of Escherichia coli isolated from pigs (n = 643), pork (n = 111) and pig carcasses (n = 110) in Thailand during 2008–2018 were included. Susceptibilities to antibiotics, disinfectants (i.e. triclosan (TCS), chlorhexidine digluconate (CHX), benzalkonium chloride (BKC), glutaraldehyde (GLU), formaldehyde (FOR)) and heavy metals (i.e. zinc chloride and copper sulfate) were examined. The E. coli isolates susceptible to all antibiotics (n = 24) were included for in vitro exposure experiment. The effect of phenylalanine arginine β-naphthylamide (PAβN) on the susceptibilities was determined. The results showed that the majority of E. coli (89%) from pigs, pig carcass and pork were multidrug resistant strains. The MICs of all disinfectants and heavy metals, except triclosan were clustered under 1 or 2 concentrations, indicating that there was no development or development at limited degree of resistance to these substances. TCS MICs for E. coli isolated in 2011–2014 were significantly higher than that of the strains in 2008–2010. The weak correlation between biocide MICs (BKC, GLU, TCS, CHX and CuSO4) and antibiotic resistance was observed (p < 0.05). Exposure to triclosan, benzalkonium chloride and chlorhexidine digluconate selected for spontaneous-resistant mutants exhibited cross resistance to at least one antibiotic. The cross resistance was observed between TCS and all eight antimicrobial drugs; BKC and chloramphenicol/ciprofloxacin/sulfamethoxazole/tetracycline; and CHX and ciprofloxacin/gentamicin/streptomycin. The presence of PAβN restored MICs of chloramphenicol and trimethoprim in some BKC- and TCS-spontaneous resistant mutants, highlighting the important role of multidrug efflux system as cross-resistance mediated mechanism. The presence of PAβN, carbonyl cyanide m-chlorophenylhydrazone (CCCP) and reserpine could not restore ciprofloxacin MIC in some ciprofloxacin-resistant mutant derivatives with no mutation in neither gyrA nor parC, suggesting the existence of proton motive force-independent mechanisms. In conclusions, the widely-used disinfectants and heavy metals play an important role as non-antibiotic selective pressure for emergence and spread of AMR. Therefore, susceptibilities to disinfectants/heavy metals should be routinely monitored.
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