Novel Fluorescent Nano Carbon Quantum Dots Derived From Lactarius Hatsudake for High Selective Vitamin B12 Detection

Abstract Background VB12 is involved in the production of bone marrow red blood cells, preventing pernicious anemia and brain nerve damage. Objective A large edible fungus Lactarius hatsudake (Lh) was used as a carbon source to produce fluorescent carbon dots (CDs) with high quantum yield and high photostability by a one-step hydrothermal method. Method The structure and optical properties of Lh-CDs were characterized by XRD, XPS, FTIR, and UV-Vis, and the stability of Lh-CDs was studied. The maximum excitation wavelength and emission wavelength of Lh-CDs were 324 and 408 nm, respectively, and the average fluorescence lifetime was 2.29 ns. Results The linear range is 0∼20 μM (R2 = 0.9972) with a detection limit of 36.9 nM. At the same time, the mechanism of fluorescence quenching was internal filtration effect. Conclusions It showed that Lh-CDS had good selectivity, high sensitivity, and good anti-interference ability to VB12, which provided a new detection method for VB12.Highlights The fluorescence intensity of the LH-CDs could be effectively quenched by VB12. Highlights The fluorescence intensity of the LH-CDs could be effectively quenched by VB12.