诱导多能干细胞
重编程
同源盒蛋白纳米
杜氏肌营养不良
细胞生物学
mdx鼠标
肌营养不良
干细胞
生物
胚胎干细胞
医学
细胞
内科学
肌营养不良蛋白
遗传学
基因
作者
Bo Wang,Yuko Miyagoe‐Suzuki,Erica Yada,Naoki Ito,Takashi Nishiyama,Miho Nakamura,Yusuke Ono,Norio Matsuki,Makoto Segawa,S. Masuda,Shin’ichi Takeda
出处
期刊:PLOS Currents
[Public Library of Science]
日期:2011-10-27
卷期号:3: RRN1274-RRN1274
被引量:53
标识
DOI:10.1371/currents.rrn1274
摘要
Induced pluripotent stem cells (iPSCs) hold promise as a potential treatment for Duchenne muscular dystrophy (DMD). To determine the impact of the donor's age on reprogramming, we generated iPSCs from muscle-derived fibroblasts (MuFs) of mdx mice aged 6 weeks, 6 months, and 14 months. MuFs from 14-month-old mdx mice showed lower proliferative activity and lower reprogramming efficiency, compared with those from younger mdx mice. Furthermore, iPSCs derived from 14-month-old mdx mice (14m-MuF-iPSCs) gradually lost Nanog expression, and regressed in conventional ES medium during passages. Interestingly, inhibition of TGF-β signaling and BMP signaling stabilized Nanog expression and promoted self-renewal of 14m-MuF-iPSCs. Finally, rescued mdx-derived iPSCs efficiently differentiated into the skeletal muscle lineage.
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