纤维连接蛋白
小岛
层粘连蛋白
细胞外基质
过剩2
基底膜
体外
细胞生物学
生物
胰岛素
内分泌学
生物化学
葡萄糖转运蛋白
作者
Jamal Daoud,Maria Petropavlovskaia,Lawrence Rosenberg,Maryam Tabrizian
出处
期刊:Biomaterials
[Elsevier]
日期:2010-03-01
卷期号:31 (7): 1676-1682
被引量:134
标识
DOI:10.1016/j.biomaterials.2009.11.057
摘要
Human islet isolation leads to the loss of the ECM basement membrane which contributes to eventual apoptosis in vitro. The reestablishment of this environment is vital in understanding the mechanism of islet interaction with its surroundings in order to arrive at conditions favourable to islet culture in vitro. In this study, we investigated the effects of the main ECM components collagen I and IV, fibronectin, and laminin on human islet adhesion, survival, and functionality. Results have provided insight into integrin-mediated effects and behaviour. Collagen I/IV and fibronectin induced adhesion, while fibronectin was the only ECM protein capable of maintaining islet structural integrity and insulin content distribution. Furthermore, islet phenotype was eventually lost, but insulin gene expression was highest in islets cultured on collagen I and IV. However, insulin release was highest on fibronectin, along with a decrease in SUR1 expression, while glucose metabolism, along with GLUT2 and GCK expression, was highest on collagen I and IV surfaces. These findings provide a basis for the future establishment of a modified three-dimensional construct for the culture of human pancreatic islets in vitro.
科研通智能强力驱动
Strongly Powered by AbleSci AI