Cross-Synergistic Interactions between Trichoderma viride and Penicillium funiculosum Cellulase

Cross-synergistic interactions were evaluated with purified enzymes from Trichoderma viride and Penicillium funiculosum cellulase. Different synergistic patterns between enzyme components were observed. Exo-exo type synergism was found to be the most effective for degrading Avicel in all cases. Exo-endo type synergism was found to be slightly less effective. Extended hydrolysis of Avicel was carried out using mixtures of purified enzyme components with the crude cellulase from a different source. Addition of -glucosidase from P. funiculosum cellulase to T. viride cellulase provided the great enhancement of Avicel hydrolysis. In addition, exoglucanase from T. viride cellulase was found to enhance P. funiculosum cellulase in degradation of Avicel. In conclusion, it was possible to enhance the hydrolysis of Avicel by altering the proportions of enzyme components by supplementing enzyme components from a different source. Different types of synergisms acted together to achieve maximum conversion.