软骨
干细胞
透明软骨
医学
脚手架
组织工程
病理
骨髓
生物医学工程
解剖
骨关节炎
细胞生物学
生物
关节软骨
替代医学
作者
Junjun Shi,Xin Zhang,Xiangzhu Zeng,Jingxian Zhu,Yu Pi,Chunyan Zhou,Yingfang Ao
出处
期刊:Orthopedics
[SLACK, Inc.]
日期:2012-05-01
卷期号:35 (5)
被引量:21
标识
DOI:10.3928/01477447-20120426-20
摘要
Focal full-thickness articular cartilage defects are challenging to repair. The purpose of this study was to find a simple, effective 1-step articular cartilage repair method. Because stem cell niches produce a microenvironment for stem cell self-renewal, proliferation, and differentiation, we integrated in situ bone marrow stem cells with an implanted poly(L-lactic-co-glycolic acid) (PLLGA) scaffold. Marrow stem cells grew and proliferated on cell-free PLLGA scaffolds, which were evaluated by scanning electronic microscopy (SEM) and Cell Counting Kit-8 (Dojindo, Kumamoto, Japan). Twenty-seven rabbits (54 knees) with large cylinder femoral trochlear cartilage defects were created and repaired with microfracture and cell-free PLLGA scaffold implantation (group 1), microfracture (group 2), or cell-free PLLGA scaffold implantation (group 3). Outcomes were evaluated by magnetic resonance imaging, International Cartilage Repair Society scores, histology, and immunohistochemistry. The repair effects were better in group 1 than in groups 2 and 3. In group 1, hyaline-like cartilage formed at week 24. Magnetic resonance imaging showed homogeneous signals as the adjacent normal cartilage. Collagen type II and toluidine blue were stained positively as normal cartilage tissue, and the color and thickness of regenerated tissue were similar to surrounding normal tissue. The combination of microfracture and cell-free PLLGA scaffold implantation used endogenous marrow stem cells in situ and promoted hyaline-like cartilage regeneration rapidly and effectively.
科研通智能强力驱动
Strongly Powered by AbleSci AI