渗透
纳米颗粒
化学
体内
生物物理学
右旋糖酐
增强子
磁导率
单层
化学工程
色谱法
纳米技术
材料科学
膜
生物化学
生物
基因
工程类
基因表达
生物技术
作者
Yuxue Cao,Taskeen Iqbal Janjua,Zhi Qu,Bastian Draphoen,Yongping Bai,Mika Lindén,Md. Moniruzzaman,Sumaira Z. Hasnain,Tushar Kumeria,Amirali Popat
出处
期刊:Biomaterials Science
[The Royal Society of Chemistry]
日期:2023-01-01
卷期号:11 (13): 4508-4521
被引量:4
摘要
Nanoparticle based permeation enhancers have the potential to improve the oral delivery of biologics. Recently, solid silica nanoparticles were discovered to improve the intestinal permeability of peptides and proteins via transient opening of the gut epithelium. In this study, we have developed small-sized (∼60 nm) virus-like silica nanoparticles (VSNP) as a reversible and next generation non-toxic permeation enhancer for oral delivery of biologics. Our results show that the anionic VSNP showed a better permeation-enhancing effect than the same sized spherical Stöber silica nanoparticles (∼60 nm) by enhancing the apparent insulin permeability by 1.3-fold in the Caco-2 monolayer model and by 1.2-fold in the Caco-2/MTX-HT-29 co-culture model. In vivo experiments in healthy mice demonstrated that anionic VSNP significantly enhanced the permeation of fluorescently labelled 4 kDa dextran after oral administration compared to Stöber nanoparticles and positively charged VSNP. The results indicated that the nanoscale surface roughness is an important consideration when designing nanoparticle-based permeation enhancers. Overall, our study shows for the first time that small-sized (∼60 nm) VSNP with nanoscale surface roughness can be used as a non-toxic permeation enhancer for oral delivery of therapeutic peptides and proteins.
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