Separation and determination of quercetin,rutin and chlorogenic acid in houttuyniae and hawthorn fruit by reverse-flow micellar electrokinetic chromatographic method

A reverse-flow micellar electrokinetic chromatographic method with direct on-column ultraviolet detection was developed for the separation and determination of quercetin,rutin and chlorogenic acid in herba houttuyniae and hawthorn fruit.The separation conditions of the three analytes were optimized with respect to the concentrations of sodium dodecyl sulfate,NaH_2PO_4, pH and applied voltage.Baseline separation was obtained for the three analytes within 15min, using a running buffer containing 70mmol/L sodium dodecyl sulfate,30mmol/L NaH_2PO_4, pH2.1 and-15kV voltage.The linear calibration range was 25～400μg/mL for quercetin (r=0.9992)and rutin(r=0.9992);12.5～200μg/mL for chlorogenic acid(r=0.9999).The recoveries were 98.5%～103.7%,95.8%～97.6%and 99.1%～103.8%for quercetin,rutin and chlorogenic acid,respectively.The detection limits of three analytes(S/N=3)were 2.52,3.1,3.64μg/mL, respectively.