Single cell electrochemistry is a challenge to electrochemistry and cell biology.In the present paper,A single yeast cell was captured on the methylene blue modified Pt disk electrode surface.The electrochemical behavior of the captured single cell was studied by cyclic voltammetry and electrochemical impedance spectroscopy.The captured single yeast cell shows an surface controlled irreversible reduction peak with the catalysis of methylene blue.Some relative parameters such as the resistances of electrode-solution,solution-cell membrane,electrochemical reaction,cell membrane-inner solution of cell,and electrical capacities were obtained from the electrochemical impedance of the single cell,which offers a basis for the further studies of cell electrochemistry.