The peroxidase isoenzyme of leaves of six species Petunia hybrida were studied by the method of polyacrylamide gelelectrophoresis and their sibship were studied by the method of index analysis. The results showed the leaves of different species of Petunia hybrida have different enzyme spectrum zone. The consanguinity of pink Petunia hybrida is closed to red Petunia hybrida,a single pind Petunia hybrida is closed to rose color Petunia hybrid and purple Petunia hybrida. but white Petunia hybrida remote to a single pind Petunia hybrida, rose color Petunia hybrid and purple Petunia hybrida.