Deep-UV hyperspectral microscopy with phase and absorption-based contrast

Ultraviolet hyperspectral interferometric (UHI) microscopy is a novel molecular imaging technique that retrieves wideband, high-resolution spectral information of the wavelength-dependent real part of the refractive index (i.e., dispersion), and the total attenuation coefficient of biological samples in the deep-ultraviolet (UV) spectral region. Here I will discuss the design of the system and processing methods used to extract the hyperspectral information. I will also present results from individual cells, tissues, and a detail study of spectral properties of several important biomolecules. Finally, biomedical applications and future directions will be discussed.