Expression on specific fragement of flagellin from Borrelia burgdorferi and its diagnostic

The purpose was to extract Borrelia burgdorferi DNA and use PCR method to obtain the gene coding of the Borrelia burgdorferi flagellin from 394 to 798 nucleotide that exhibited low homology with related gene from other bacterial specises we insert Borrelia burgdorferi flagellin from 394 to 798 nucleotide to PGEX-4T-1 vector,verified by DNA sequence detection and tren thansformed it into in E.coli BL 21(DE3) to induce target protein.Then we purified the recombination protein as ELISA antigen to diagnosis Lyme disease.On other hand,we infected mouse with Borrelia burgdorferi.Two weeks,we got positive serum from mouse.We used recombinant protein as diagnostic antigen andpositive mouse serum as antibody to conduct ELISA test and analysis.The ELISA results showed the feasibility of recombinant protein as diagnostic antigen to diagnose Lyme disease,which lay foundations for the diagnosis of Lyme disease.