Effect of Hirudin on Human Hypertrophic Scar Fibroblast

Objective To explore the effect of hirudin on the apoptosis of the human hypertrophic scar fibroblast(HSFb)cultivated in vitro.Methods Experiment of isolate cultivating HSFb and cells after 4-7 generations were used in subsequent experiments.The expression of Bax and Bcl-2 in HSFb were analyzed by Western blot.Results Hirudin had an inhibitive effect on the proliferation of HSFb;Immunohistochemistry and Western blot results showed that hirudin increased the expression of Bax,but inhibited the expression of Bcl-2,compared with the control group,the difference was statistically significant(P0.05).Conclusion Hirudin of experimental concentration has significant inhibitory effects on HSFb.