Research of siRNA transfection efficiency

Objective: To evaluate the transfection efficiency and optimal conditions of siRNA.Methods:Synthysized siRNA were transfected into human umbilical vein endothelial cells,we identified the rate of the transfection by fluorescent microscope and Western blotting.Results:The transfection rate of 35% was attained when FITC labeled negative siRNA was transfected into cells with siPORT~TM NeoFX~TM.Conclusion:The transfection activity of siRNA is observed,which provides a basis for finding the better method to check the rate of the transfection.