Determination of Profenofos in Water Using Dispersive Liquid-Liquid Microextraction-High Performance Liquid Chromatography

A method was developed for the determination of profenofos in water by dispersive liquid-liquid microextraction(DLLME)-high performance liquid chromatography(HPLC).Some experimental parameters that influence extraction efficiency,such as the kind and volume of disperser solvents and extraction solvents,extraction time,addition of salt and PH,were investigated.The optimal experimental conditions were as follows:approximately 15μL of trichloroethane(extraction solvents)and 750μL of acetonitrile(disperser solvents)were added to the water samples,then set at room temperature for 2 min,centrifugation at 3000 r/min for 2 min.Finally,3μL of sedimented phase was injected into the HPLC for analysis.Under the optimum conditions,the enrichment factors of the profenofos was about 270,the detection limits was 0.002 mg/L.The recoveries for standard addition fell in the range from 86.9%to 118%and the relative standard deviations(RSD)were between 1.4%and 6.1%.The obtained results showed that the DLLME-HPLC method simple and low-cost.