A Method of Establishing Experimental Model of Thoracolumbar Anterior Dural Injury Cerebrospinal Fluid Leakage in Rabbit

Objective To establish a long-term surviving model of thoracolumbar anterior dural injury cerebrospinal fluid leakage in rabbit. Methods Establish an animal model which include 32 rabbits. Those animals were randomly divided into four groups with 8 rabbits each. After Ketamine Anesthesia,adopt abdomino-thoracic incision,burst through musculi psoas,removal 12th rib,expose side wall of T12-L2 verte- bra,truncate and ligate L1 arteriae lumbales. Subtotal resect L1 vertebrae,expose dural mater. Make different thoracolumbar dural mater injury in each groups. Group A:simplicity linear dural mater incision of 0.5cm,Group B:simplicity linear dural matter incision of 1cm,Group C:dural mater defect of 0.5cm2,GroupD:dural mater defect of 1cm2. gelfoam and hemostatic gauze were used to wadded the dural mater leakage,BFS is used too. All animals have cerebrospinal fluid from drainage tube. 30 days after surgery. CT spinal cannal myelography,methylene blue test, and dural matter pathologic examination were tested. Results Group A:all rabbits dural matter wound healed. Group B:4 cases dural mater healed,1 case found meningeal cyst,1 case dural mater incision reduce. Group C:2 case dural matter healed,3 cases dural mater incision re- duce,2 case found meningeal cyst. Group D:5 cases dural mater incision reduce,1 case found meningeal cyst. In each group,we can see homeocyte,phlogocyte etc inflammatory cell infiltrate,dural mater thicken. non-necrotic inflammatory infiltration,dural mater incision cicatriza- tion. Conclusion It is safe,convenient and credible to establish a model of thoracolumbar anterior dural injury cerebrospinal fluid leakage in rabbit by adopting abdominothoracic incision,removing 12th rib,exposing side wall of T12-L2 vertebra,truncating and ligating L1 arteriae lum- bales,subtotal resecting L1 vertebrae,exposing dural mater and making different thoracolumbar dural matter injury in rabbit. It can provide an experimental foundation to subsequent research.