光谱学
激发态
发射光谱
荧光
时间分辨光谱学
荧光光谱法
化学
动力学
分析化学(期刊)
谱线
分子物理学
原子物理学
物理
光学
量子力学
天文
色谱法
作者
Sri Rama Koti Ainavarapu,N. Periasamy
摘要
Time resolved emission spectroscopy (TRES) provides information on the excited state kinetics and heterogeneity of emissive species in a system. Time resolved area normalized emission spectroscopy (TRANES), an extension to TRES, is a novel, model-free method for the analysis of intrinsic or extrinsic fluorescence probes in complex chemical and biophysical systems [Koti, Krishna, and Periasamy, J. Phys. Chem. A 105, 1767 (2001)]. Observation of a single isoemissive point in TRANES analysis of fluorescence is an unambiguous indication of the presence of two emissive species in the system. The presence of multiple isoemissive points in TRANES spectra is confirmed using simulation and experimental data of multicomponent systems.
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