Ultrasonic extraction and identification of carp scale collagen

Abstract In this experiment, the carp scale as raw material, containing calcium and protein impurities in the scale and so on, respectively by two sodium EDTA (EDTA two sodium, EDTA) and sodium chloride solution (sodium chloride, NaCl) solution and its removal, scale collagen protein extraction and design. By using different single factor test the 4 factors determine the extraction of collagen during the experiment the optimum extraction process, the 4 factors are the enzyme (pepsin) dosage, ultrasonic time, solid-liquid ratio, extraction liquid (acetic acid) concentration of glue. The optimum extraction process for raw protein ultrasonic time 200Min, enzyme dosage 300mg/g, extraction concentration of 0.3 mol/L, the ratio of material to liquid 1:40 (g/mL), in this condition, the extraction rate was 28.7. By UV spectrophotometer identification, the material in the 230nm have obvious absorption peak, so the test the material of collagen.