Comparative Analysis of Paddy Soil Denitrifying Bacteria with Soil Phospholipid Fatty Acid Profile

To characterize the metabolic versatility of denitrifying bacterial communities residing in the paddy soil, phospholipid fatty acid (PLFA) analyses were done along with nosZ encoding nitrous oxide reductase gene-based PCR-DGGE and real-time Q-PCR analysis. In this study, we have analyzed the abundance of nitrous oxide reductase (nosZ) genes and DGGE-based denitrifier community assessment was found to be correlated with soil PLFA profile in terms of their pattern analysis. Among all detected denitrifying bacteria, Ochrobactrum sp. represented a major fraction following Cupriavidus and uncultured bacteria. The data related to nosZ gene abundance were found to show similar pattern with PLFA-based lipid profile. Greater amount of lipid content (C18-C20) and denitrifiers diversity was apparently visible at Chandauli of Eastern UP, India and thus positively impact each other in the soil. Our study suggests that the nosZ-based DGGE and gene abundance data were increased in response to soil PLFA profiles. Subsequently, the presence of an increased lipid content of soil samples with increased abundance and diversity of denitrifying bacteria could be helpful in managing nutrient dynamics as well as microbial dynamics of paddy soil ecosystem.