Role of mast cells in Staphylococcus aureus enterotoxin Binduced atopic dermatitis-like skin inflammation in mice

湿疹面积及严重程度指数 卵清蛋白 特应性皮炎 真皮 医学 染色 免疫学 皮肤活检 肥大细胞 免疫组织化学 病理 炎症 免疫球蛋白E 免疫系统 活检 抗体
作者
Shan Wang,Zuotao Zhao,Yuhan Wang,Ping Tu
出处
期刊:Chinese Journal of Dermatology [Hospital for Skin Diseases, Institute of Dermatology, Chinese Academy of Medical Sciences]
卷期号:51 (1): 26-30
标识
DOI:10.3760/cma.j.issn.0412-4030.2018.01.007
摘要

Objective To investigate the role of mast cells in Staphylococcus aureus enterotoxin B (SEB) -induced atopic dermatitis (AD) -like skin inflammation in BALB/c mice. Methods A total of 24 BALB/c mice were randomly and equally divided into 4 groups to be topically treated with ovalbumin (OVA group) , SEB (SEB group) , OVA+ SEB (OVA+ SEB group) and sodium chloride physiological solution (control group) respectively, so as to establish mouse models of epicutaneously induced AD-like skin inflammation. The AD-like skin lesions were evaluated by clinical observation and eczema area and severity index (EASI) . Biopsy specimens were obtained from lesional skin of mice and then subjected to toluidine blue staining and immunohistochemical staining to count the mast cells, observe the morphology and distribution of mast cells, and calculate the percentage of degranulated mast cells. Results After 7-week treatment, the OVA group, SEB group and OVA+ SEB group all showed severer local skin inflammation, higher EASI scores and denser infiltration of inflammatory cells compared with the control group. Moreover, the OVA+ SEB group showed significantly severer local skin inflammation, skin lesions and degree of infiltration of inflammatory cells compared with the OVA group and SEB group (all P < 0.05) . The number of mast cells in the dermis of AD-like skin lesions per high-power field (× 400) was significantly higher in the OVA group (median[quartile range]: 10.625[3.675]) , SEB group (11.000[4.163]) and OVA+ SEB group (13.875[8.813]) than that in the control group (5.925[2.088], all P < 0.05) . The SEB group (71.083% ± 14.519%) and OVA+ SEB group (58.767% ± 16.978%) both showed significantly higher percentage of degranulated mast cells compared with the OVA group (24.050% ± 11.161%, both P < 0.05) and control group (23.617% ± 8.132%, both P < 0.05) . Bivariate correlation analysis showed that the number of mast cells in the skin lesions was positively linearly correlated with the EASI scores (P < 0.05) . Conclusions Epicutaneous application of SEB can induce AD-like skin lesions in mice, and can exacerbate the severity of OVA-induced AD-like skin lesions. Mast cell proliferation, activation/degranulation and tryptase release may participate in the inflammation. Key words: Dermatitis, atopic; Mast cells; Staphylococcus aureus; Enterotoxins; Tryptases; Ovalbumin
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
小达完成签到 ,获得积分10
4秒前
可靠的书桃完成签到 ,获得积分10
10秒前
dhjic完成签到 ,获得积分10
20秒前
ken131完成签到 ,获得积分10
20秒前
yinyin完成签到 ,获得积分10
22秒前
希子完成签到 ,获得积分10
23秒前
jxwe完成签到 ,获得积分10
27秒前
蓝景轩辕完成签到 ,获得积分0
27秒前
小绵羊完成签到,获得积分20
31秒前
666星爷完成签到,获得积分10
31秒前
kk应助科研通管家采纳,获得10
32秒前
32秒前
天天快乐应助时尚东蒽采纳,获得10
35秒前
37秒前
明理丹烟应助philophysics采纳,获得10
39秒前
彩色的冷梅完成签到 ,获得积分10
46秒前
华夫饼完成签到 ,获得积分10
50秒前
50秒前
mia发布了新的文献求助10
51秒前
缓慢的甜瓜完成签到 ,获得积分10
51秒前
午午午午完成签到 ,获得积分10
52秒前
地狱跳跳虎完成签到 ,获得积分10
54秒前
56秒前
温婉的夏烟完成签到 ,获得积分10
57秒前
58秒前
5High_0完成签到 ,获得积分10
1分钟前
时尚东蒽发布了新的文献求助10
1分钟前
洁净青筠完成签到,获得积分10
1分钟前
ommphey完成签到 ,获得积分10
1分钟前
荔枝完成签到 ,获得积分10
1分钟前
木木杉完成签到 ,获得积分10
1分钟前
chenying完成签到 ,获得积分0
1分钟前
时尚东蒽完成签到,获得积分20
1分钟前
遇楹完成签到 ,获得积分10
1分钟前
内向南风完成签到 ,获得积分10
1分钟前
雷九万班完成签到 ,获得积分10
1分钟前
PM2555完成签到 ,获得积分10
1分钟前
单兰完成签到 ,获得积分10
2分钟前
竞鹤发布了新的文献求助10
2分钟前
mousehe完成签到,获得积分10
2分钟前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Cognitive linguistics critical concepts in linguistics 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
氟盐冷却高温堆非能动余热排出性能及安全分析研究 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3052652
求助须知:如何正确求助?哪些是违规求助? 2709874
关于积分的说明 7418298
捐赠科研通 2354492
什么是DOI,文献DOI怎么找? 1246104
科研通“疑难数据库(出版商)”最低求助积分说明 605951
版权声明 595921