荧光
铜
荧光团
检出限
选择性
化学
皮兰
光化学
活细胞
分析化学(期刊)
催化作用
生物化学
立体化学
生物
生物系统
色谱法
物理
有机化学
量子力学
作者
Zheng Li,Yanqi Xu,Huandi Xu,Mengyuan Cui,Tianguang Liu,Xiangyu Ren,Jianfei Sun,Dawei Deng,Yueqing Gu,Peng Wang
标识
DOI:10.1016/j.saa.2020.118819
摘要
Copper (Cu) plays a significant role in the process of oxygenic photosynthesis in living systems. The detection of copper ion (Cu2+) is valuable and meaningful for further investigating the functions of Cu2+ under physiological and pathological conditions. In this paper, a novel fluorescence probe DCM-Cu based on the near-infrared (NIR) fluorophore dicyanomethylene-4H-pyran (DCM) was designed for Cu2+ detection. The probe DCM-Cu possessed characteristic of “turn-on” fluorescent signal in the presence of Cu2+ through the enhanced ICT process. It exhibited satisfactory sensitivity and selectivity toward Cu2+. A good linear correlation was observed between the concentrations of Cu2+ and the fluorescence intensities at 700 nm. The detection limit (LOD) of DCM-Cu toward Cu2+ was calculated to be 2.54 × 10−8 M. Importantly, DCM-Cu was successfully applied in the detection of Cu2+ in living MCF-7 cells and tumor tissue with low cytotoxicity. Therefore, this probe would have the potential to monitor cellular Cu2+ in the living system and be applied to the diagnosis of related diseases.
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