[Clinical significance of interleukin-6 and -8 in patients with chronic periodontal disease and acute exacerbation of chronic obstructive pulmonary disease].

Objective: To investigate the serum levels of interleukin (IL)-6 and -8 in patients with chronic periodontal disease and acute exacerbation of chronic obstructive pulmonary disease (AECOPD) and the possible relationship between IL-6 and IL-8 with two diseases. Methods: A total of 40 cases of healthy subjects (control group 1) from graduate school of Anhui Medical University, and 120 cases (40 cases in each of the 3 groups) of eligible patients were collected, of which 40 were patients with chronic periodontal disease and AECOPD (experimental group) from Department of Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University and Anhui NO.2 Provincial People's Hospital, 40 were patients with chronic periodontal disease (control group 2) from Department of Stomatology, The First Affiliated Hospital of Anhui Medical University, 40 were patients with AECOPD (control group 3) from Department of Respiratory Medicine, The First Affiliated Hospital of Anhui Medical University. The clinical indicators of all subjects were collected, including tooth mobility degree, probing depth (PD), bleeding index (BI), attachment level (AL), vital capacity max (VC Max), forced expiratory volume in first second (FEV1) and forced expiratory volume in first second to forced vital capacity (FEV1/FVC) ratio. Enzyme linked immunosorbent assay (ELISA) was used to detect the serum levels of IL-6 and IL-8 in the subjects of four groups. Results: The attachment levels had no significant differences between experimental group and control group 2 (P>0.05). The pulmonary function indices of experimental group including VC MAX% pre[(56.1±11.1)%], FEV1 %pre [(44.8±12.2)%], FEV1/FVC(%) [(56.8±11.4)%] were significantly different from those in control group 3 [(66.3±10.1)%, (53.0±10.4)%, (66.5±8.2)%, respectively]. The IL-6 levels of experimental group, control groups 1, 2 and 3 were (14.4±3.9), (2.1±1.1), (4.8±1.9) and (8.6±1.4) ng/L, respectively. And the IL-8 levels were (35.3±33.3), (4.8±1.7), (9.7±3.3) and (15.6±9.6) ng/L. In experimental group the IL-6 and IL-8 levels were significantly higher than those in control groups 1, 2, and 3 (P<0.01). In control group 2 and 3 the IL-6 and IL-8 levels were significantly higher than that in control group 1 (P< 0.01). Conclusions: The IL-6 and IL-8 levels of experimental group were significantly increased. IL-6 and IL-8 may be associated with the development of periodontal disease and AECOPD closely.目的： 研究慢性牙周炎（chronic periodontitis ，CP）及慢性阻塞性肺疾病急性加重（acute exacerbation of chronic obstructive pulmonary disease，AECOPD）患者血清白介素（interleukin，IL）6和IL-8水平，并初步探讨IL-6和IL-8与两种疾病的关系。 方法： 选取安徽医科大学第一附属医院呼吸内科及安徽省第二人民医院呼吸内科符合条件的40例AECOPD伴CP患者为试验组（AECOPD+CP组）；40名安徽医科大学研究生学院健康者为对照1组（健康对照组）；安徽医科大学第一附属医院口腔科符合条件的40例单纯CP患者为对照2组（CP组）；安徽医科大学第一附属医院呼吸内科符合条件的40例单纯AECOPD患者为对照3组（AECOPD组）。采集所有研究对象的临床指标，包括牙齿松动度、探诊深度、出血指数、附着丧失以及最大肺活量、第一秒用力呼气容积（forced expiratory volume in first second, FEV1）、FEV1与用力肺活量（forced vital capacity，FVC）的比值（FEV1/FVC），并使用酶联免疫吸附测定法检测各组研究对象血清中IL-6及IL-8的含量。 结果： AECOPD+CP组各项牙周指标（探诊深度、出血指数、附着丧失）与CP组相比差异均无统计学意义（P>0.05）；AECOPD+CP组肺功能指标[最大肺活量占预计值百分比、FEV1占预计值百分比、FEV1/FVC值分别为（56.1±11.1）%、（44.8±12.2）％和（56.8±11.4）%]均显著低于AECOPD组[最大肺活量占预计值百分比、FEV1占预计值百分比、FEV1/FVC值分别为（66.3±10.1）%、（53.0±10.4）％和（66.5±8.2）%]（P<0.01）；AECOPD+CP组血清IL-6与IL-8水平[分别为（14.4±3.9）、（35.3±33.3）ng/L]均显著高于其他三组（P<0.01）；CP组和AECOPD组血清IL-6与IL-8水平均显著高于健康对照组（P<0.01）。 结论： AECOPD伴CP患者血清IL-6和IL-8水平显著升高，IL-6和IL-8可能与CP和AECOPD的发生发展存在密切关系。.