生物分子
显微镜
生物标本
纳米尺度
纳米技术
生物材料
显微镜
生物成像
材料科学
生物
生物系统
物理
光学
荧光
作者
Asmamaw T. Wassie,Yongxin Zhao,Edward S. Boyden
出处
期刊:Nature Methods
[Springer Nature]
日期:2018-12-20
卷期号:16 (1): 33-41
被引量:296
标识
DOI:10.1038/s41592-018-0219-4
摘要
Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial resolution. We recently discovered that preserved biological specimens can be physically expanded in an isotropic fashion through a chemical process. Expansion microscopy (ExM) allows nanoscale imaging of biological specimens with conventional microscopes, decrowds biomolecules in support of signal amplification and multiplexed readout chemistries, and makes specimens transparent. We review the principles of how ExM works, advances in the technology made by our group and others, and its applications throughout biology and medicine.
科研通智能强力驱动
Strongly Powered by AbleSci AI