Combination of mixed mode dispersive solid phase extraction with magnetic ionic liquids based dispersive liquid–liquid microextraction for the extraction of anticoagulant drugs from urine samples

• A combination of DSPE and DLLME was developed for the extraction of DOACs. • A ternary mixture of sorbents was used in DSPE for the effective adsorption of DOACs. • HPLC-DAD was used for determination of the analytes. • LODs and LOQs were achievable at ng mL –1 level. • Greenness, simplicity, and being effective were advantages of the method. In this report, combination of a mixed mode dispersive solid phase extraction with dispersive liquid–liquid microextraction has been performed in order to the effective isolation of three anticoagulant drugs (anagrelide, betrixaban, and apixaban) from urine sample. The extracted drugs were quantified by high performance liquid chromatography–diode array detector. Firstly, analytes were adsorbed onto a mixture of octadecylsilane, graphitized carbon black, and primary secondary amine sorbents which their composition was obtained by a simplex centroid design. To increase the adsorption efficiency, sonication of the solution and sorbents mixture was performed. The loaded analytes onto the sorbent surface were washed employing a water–miscible organic solvent which was utilized as a disperser in microextraction process. The microextraction step was done using a magnetic ionic liquid (as an extractant) and the droplets were sedimented at the bottom of tube with the aid of an external magnetic field. Satisfactory analytical results such as wide linear ranges (0.21–250 µg/L), high enrichment factors (223–247) and extraction recoveries (76–84%), and low limits of detection (0.04–0.06 µg/L) and quantification (0.16–0.21 µg/L) were obtained using the optimum experimental situations. Lastly, the offered procedure was utilized for the quantification of the opted analytes in urine samples.