萜类
农杆菌
瞬态(计算机编程)
生物
生产(经济)
植物
转化(遗传学)
计算机科学
生物化学
基因
宏观经济学
经济
操作系统
作者
Jacob D. Bibik,Abigail E. Bryson,Björn Hamberger
出处
期刊:Methods in molecular biology
日期:2024-01-01
卷期号:: 21-34
标识
DOI:10.1007/978-1-0716-3658-9_2
摘要
As the field of plant synthetic biology continues to grow, Agrobacterium-mediated transient expression has become an essential method to rapidly test pathway candidate genes in a combinatorial fashion. This is especially important when elucidating and engineering more complex pathways to produce commercially relevant chemicals like many terpenoids, a widely diverse class of natural products of often industrial relevance. Agrobacterium-mediated transient expression has facilitated multiplex expression of recombinant and modified enzymes, including synthetic biology approaches to compartmentalize the biosynthesis of terpenoids subcellularly. Here, we describe methods on how to deploy Agrobacterium-mediated transient expression in Nicotiana benthamiana to rapidly develop terpenoid pathways and compartmentalize terpenoid biosynthesis within plastids, the cytosol, or at the surface of lipid droplets.
科研通智能强力驱动
Strongly Powered by AbleSci AI