生物
枯草芽孢杆菌
拉伤
黄曲霉毒素
基因
漆酶
16S核糖体RNA
几丁质酶
酶
微生物学
生物化学
细菌
食品科学
遗传学
解剖
作者
Meei Li Huang,Jinjie Guo,Yushan Jia,Chengshui Liao,Lei He,Jing Li,Ying Wei,Songbiao Chen,Jian Chen,Ke Shang,Rong Guo,Ke Ding,Yu Zhao
出处
期刊:Biology
[MDPI AG]
日期:2023-08-31
卷期号:12 (9): 1195-1195
标识
DOI:10.3390/biology12091195
摘要
As a class I carcinogen, aflatoxin can cause serious damage to various tissues and organs through oxidative stress injuries. The liver, as the target organ of AFB1, is the most seriously damaged. Biological methods are commonly used to degrade AFB1. In our study, the aflatoxin B1-degrading strain ZJ20 was screened from AFB1-contaminated feed and soil, and the degradation of AFB1 by ZJ20 was investigated. The whole genome of strain ZJ20 was analyzed, revealing the genomic complexity of strain ZJ20. The 16S rRNA analysis of strain ZJ20 showed 100% identity to Bacillus subtilis IAM 12118. Through whole gene functional annotation, it was determined that ZJ20 has high antioxidant activity and enzymatic activity; more than 100 CAZymes and 11 gene clusters are involved in the production of secondary metabolites with antimicrobial properties. In addition, B. subtilis ZJ20 was predicted to contain a cluster of genes encoding AFB1-degrading enzymes, including chitinase, laccase, lactonase, and manganese oxidase. The comprehensive analysis of B. subtilis provides a theoretical basis for the subsequent development of the biological functions of ZJ20 and the combinatorial enzyme degradation of AFB1.
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