Genome-wide identification and analysis of petal color related MYB transcription factor gene in Gerbera hybrida

Gerbera hybrida features petals rich in anthocyanins, which are recognized as valuable sources of colorants, antioxidants, and natural coatings. The petals of the G.hybrida cultivar ‘Hongyun’ exhibit a distinct bi-color pattern, although the underlying mechanism remains unclear. Recent research has highlighted the crucial role of MYB transcription factors in anthocyanin biosynthesis and petal pigmentation. This study presents a comprehensive analysis of the G.hybrida MYB gene family, identifying a total of 319 GhMYB genes, which include 165 MYB-related genes, 147 R2R3-MYB genes, 6 3R-MYB genes, and 1 4R-MYB gene. We further examined the gene structure, frequency of conserved sites, collinear relationships, and predictions of cis-acting elements within the R2R3-MYB subfamily, which is closely associated with flower color regulation. Expression profiles of anthocyanin synthesis genes and R2R3-GhMYBs were constructed for the upper and lower layers of the petals of 'Hongyun' at various coloring stages. Notably, two genes, GhMYB114 and GhMYB24 , exhibited significant differential expression levels and are believed to be responsible for the bi-color petal formation. Additionally, RT-qPCR confirmed the expression patterns of these related genes. Our research establishes a solid foundation for the functional study of MYB genes, offering candidate genes for future investigations into the regulation of flower color distribution in G.hybrida . • Identification and analysis of the MYB family in Gerbera hybrida on a genome scale. • Analysis of pigment formation mechanisms in bicolor G.hybrida flowers. • Construction of a dynamic expression atlas of R2R3-MYBs in bicolor G.hybrida flowers. • Discovery of key genes regulating bicolor flower coloration patterns in G.hybrida .