胶质细胞源性神经生长因子
脊髓
椎板切除术
脐带
医学
脊髓损伤
川地34
脐带血
末端脱氧核苷酸转移酶
神经营养因子
病理
免疫学
内科学
标记法
生物
免疫组织化学
干细胞
细胞生物学
受体
精神科
作者
Cheng-Hsing Kao,Sheng-Hsien Chen,Chung-Ching Chio,Mao‐Tsun Lin
出处
期刊:Shock
[Ovid Technologies (Wolters Kluwer)]
日期:2008-01-01
卷期号:29 (1): 49-55
被引量:61
标识
DOI:10.1097/shk.0b013e31805cddce
摘要
Human umbilical cord blood-derived CD34+ cells were used to elucidate the mechanisms underlying the beneficial effects exerted by cord blood cells in spinal cord injury (SCI). Rats were divided into four groups: (1) sham operation (laminectomy only); (2) laminectomy + SCI + CD34− cells (5 × 105 human cord blood lymphocytes and monocytes that contained <0.2% CD34+ cells); (3) laminectomy + SCI + CD34+ cells (5 × 105 human cord blood lymphocytes and monocytes that contained ∼95% CD34+ cells); and (4) laminectomy + SCI + saline (0.3 mL). Spinal cord injury was induced by compressing the spinal cord for 1 min with an aneurysm clip calibrated to a closing pressure of 55 g. CD34 cells or saline was administered immediately after SCI via the tail vein. Behavioral tests of motor function measured by maximal angle an animal could hold to the inclined plane were conducted at days 1 to 7 after SCI. The triphenyltetrazolium chloride staining and terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end labeling assay were also conducted after SCI to evaluate spinal cord infarction and apoptosis, respectively. To elucidate whether glial cell line-derived neurotrophic factor (GDNF) or vascular endothelial growth factor (VEGF) can be secreted in spinal cord-injured area by the i.v. transplanted CD34+ cells, analysis of spinal cord homogenate supernatants by specific enzyme-linked immunosorbent assay for GDNF or immunofluorescence for VEGF was conducted. It was found that systemic administration of CD34+, but not CD34−, cells significantly attenuated the SCI-induced hind limb dysfunction and spinal cord infarction and apoptosis. Both GDNF and VEGF could be detected in the injured spinal cord after transplantation of CD34+, but not CD34−, cells. The results indicate that CD34+ cell therapy may be beneficial in reversing the SCI-induced spinal cord infarction and apoptosis and hindlimb dysfunction by stimulating the production of both VEGF and GDNF in a spinal cord compression model.
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