过程性
增殖细胞核抗原
DNA聚合酶δ
DNA聚合酶
DNA复制
DNA钳
生物
分子生物学
聚合酶
复制因子C
化学
DNA
DNA聚合酶Ⅱ
蛋白质亚单位
遗传学
基因
真核细胞DNA复制
聚合酶链反应
逆转录酶
作者
Yujue Wang,Qian Zhang,Huiqing Chen,Xiao Li,Weijun Mai,Keping Chen,Sufang Zhang,Ernest Y. Lee,Marietta Y.W.T. Lee,Yajing Zhou
出处
期刊:PLOS ONE
[Public Library of Science]
日期:2011-11-02
卷期号:6 (11): e27092-e27092
被引量:17
标识
DOI:10.1371/journal.pone.0027092
摘要
Mammalian DNA polymerase δ (pol δ), a four-subunit enzyme, plays a crucial and versatile role in DNA replication and various DNA repair processes. Its function as a chromosomal DNA polymerase is dependent on the association with proliferating cell nuclear antigen (PCNA) which functions as a molecular sliding clamp. All four of the pol δ subunits (p125, p50, p68, and p12) have been reported to bind to PCNA. However, the identity of the subunit of pol δ that directly interacts with PCNA and is therefore primarily responsible for the processivity of the enzyme still remains controversial. Previous model for the network of protein-protein interactions of the pol δ-PCNA complex showed that pol δ might be able to interact with a single molecule of PCNA homotrimer through its three subunits, p125, p68, and p12 in which the p50 was not included in. Here, we have confirmed that the small subunit p50 of human pol δ truthfully interacts with PCNA by the use of far-Western analysis, quantitative ELISA assay, and subcellular co-localization. P50 is required for mediation of the interaction between pol δ subassemblies and PCNA homotrimer. Thus, pol δ interacts with PCNA via its four subunits.
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