[Mechanism Underlying the Inhibitory Effect of MiR-532-3p on the Cells Proliferation of Diffuse Large B-Cell Lymphoma].

To investigate the effects and underlying mechanism of miR-532-3p and resibufogenin (RES) by regulating Wnt/β-catenin signaling on diffuse Large B-cell lymphoma (DLBCL) cells proliferation.DLBCL tissues and adjacent normal tissues were collected from patients had been diagnosed with DLBCL at the First Hospital of Lanzhou University from October 2019 to October 2021. Four groups including mimics-NC, miR-532-3p mimics, RES control and RES treatment in SU-DHL-4 cells were designed. The expression level of miR-532-3p was detected by RT-qPCR. The protein content of β-catenin was detected by Western blot. MTT assay was used to detect the proliferation activity of SU-DHL-4 cells.miR-532-3p expression was significantly decreased in DLBCL tissues compared with adjacent normal tissues (P<0.001). The miR-532-3p content in lymphoma cells was significantly lower than that in normal lymphocytes (P<0.001). After overexpression of miR-532-3p, the viability of SU-DHL 4 cells was significantly decreased (P<0.001), with a reduced expression of β-catenin (P<0.05). RES treatment inhibited the proliferation of SU-DHL-4 cells and decreased β-catenin expression in SU-DHL-4 cells compared with the control group.Overexpression of miR-532-3p reduced Wnt/β-catenin signaling and inhibited the proliferation of lymphoma cells. Moreover, RES treatment inhibited lymphoma cells growth partially through Wnt/β-catenin signaling suppression.MiR-532-3p抑制弥漫性大B细胞淋巴瘤细胞增殖的机制研究.研究miR-532-3p和脂蟾毒甙元（RES）通过调节Wnt/β-catenin信号对弥漫性大B细胞淋巴瘤（DLBCL）细胞活性的作用机制.收集2019年10月至2021年10月兰州市兰州大学第一医院DLBCL患者的癌组织样本及癌旁组织样本；将SU-DHL-4细胞分为mimics-NC组、miR-532-3p mimics组及对照组、脂蟾毒甙元组（RES组）。采用RT-qPCR法检测miR-532-3p的表达水平；Western blot检测β-catenin的蛋白含量；MTT法检测SU-DHL-4细胞的增殖活性.相比于癌旁淋巴组织，淋巴癌组织中的miR-532-3p表达明显降低（P<0.001）；淋巴瘤细胞中的miR-532-3p的含量显著低于正常淋巴细胞（P<0.001）。过表达miR-532-3p后，SU-DHL-4细胞的增殖活性明显下降（P<0.01），而β-catenin的表达亦显著减少（P<0.05）。相比于对照组，RES抑制了SU-DHL-4细胞的增殖，同时降低了细胞中β-catenin的表达.过表达miR-532-3p降低Wnt/β-catenin信号，并抑制淋巴瘤细胞的增殖；RES可通过抑制Wnt/β-catenin的表达，降低淋巴瘤细胞的增殖活性.