微生物学
蛋白酶
生物
大肠杆菌
炎症性肠病
抗生素耐药性
溃疡性结肠炎
抗生素
抗菌剂
抗菌肽
发病机制
免疫学
基因
疾病
医学
酶
病理
遗传学
生物化学
作者
Youn Hee Cho,Michael J Renouf,Oluwafikemi Omotoso,Joseph B. McPhee
出处
期刊:Fems Microbiology Letters
[Oxford University Press]
日期:2022-01-01
卷期号:369 (1)
被引量:2
标识
DOI:10.1093/femsle/fnac098
摘要
Adherent-invasive Escherichia coli (AIEC) are isolated from inflammatory bowel disease (IBD) patients at a higher rate than from control patients. Using a collection of E. coli strains collected from Crohn's disease (CD), ulcerative colitis (UC), or non-IBD control patients, antibiotic and resistance to the antimicrobial peptides HBD-3 and LL-37 was assessed. Carriage of bacterial-encoded omptin protease genes was assessed by PCR and omptin protease activity was measured using a whole-cell based fluorescence assay. Elevated resistance to antibiotics and host defense peptides in IBD-associated AIEC were observed. IBD-associated strains showed increased (but statistically non-significant) antibiotic resistance. CD-associated strains showed greater (but statistically non-significant) resistance to HBD3-mediated killing while UC-associated strains showed statistically greater resistance to LL-37 mediated killing. High-level resistance to LL-37 was associated with carriage of omptin protease genes and with increased omptin protease activity. Antimicrobial host defense peptide resistance may be an adaptive feature of AIEC leading to enhanced pathogenesis during the initiation or progression of IBD.
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