自愈水凝胶
纳米纤维
肽
自组装肽
生物物理学
群(周期表)
材料科学
自组装
自愈
刺激
化学
纳米技术
化学工程
高分子化学
生物化学
心理学
神经科学
有机化学
替代医学
病理
医学
工程类
生物
作者
Yixuan Pang,Weihan Zhang,Yuze Zhao,Honggang Hao,Huibin Wang,Jun Liang
标识
DOI:10.1016/j.colsurfa.2023.133118
摘要
Peptides as unique supramolecular nanofibrous materials are favored choices to fabricate hydrogel for artificial extracellular matrices. Herein, we devised an amphiphilic polypeptide that could be motivated by multiple substances with positively charged group at physiological temperature and pH to assemble supramolecular nanofiber hydrogels since the electrostatic repulsion between monomers was attenuated. The hydrogelation phenomenon and spectroscopy were compatible with molecular dynamics simulations (MDs) results for revealing the assembly mechanism of this peptide, confirming that the behavior of assembling and the typical secondary structure of the assemblies was β-sheet. The impact of peptide concentration on the mechanical properties of hydrogels were described using rheology. We found that the peptide formed elastic gels from 0.3 wt% onwards in practical use and that these recovered by themselves after shear thinning that indicated they were injectable hydrogels. The successful establishment of cell spheres with high cell viability during 15 days culture in 3D space demonstrated the nanofiber hydrogels had potential in cell model construction and microtissue culture. Overall, the superior biologically friendly gel-forming conditions and excellent biological properties of this supramolecular assembly peptide material have invigorated the progress of biomedical fields such as tissue regeneration based on in vitro 3D culture.
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