Factors Influencing Agrobacterium-mediated Plant Transformation

Agrobacterium-mediated technique is a kind of methods in transgenic research used mostly for plants,and having some advantages,such as the low transformation cost,the high efficiency,being able to transfer longer gene fragments.Many factors influence transformation efficiency in the transformation pro-cess,including explant type,plant genotype,explant processing,type of bacterial strain and plasmid carrier,preculture medium composition and so on.