Gene and cell therapy

This chapter introduces the basic principle, up-to-date overview, and clinical application of gene and cell therapy. It starts with a preface with a brief introduction on gene and cell therapy about their definition, difference, and correlation. The second part is devoted to gene therapy, focusing on gene therapy strategies (replacement, addition, silencing, and editing), viral and nonviral delivery technologies, and most recent developments on human gene therapy. The third part is dedicated to cell therapy, involving sources of stem cells, summary of current stem cell-based therapy and immune cell-based therapy, and the advances in clinical application. By the end of the chapter a full discussion of progress and perspective is included to illustrate the lessons, ethical issues, and marketing of gene and cell therapy.