Principles of antibody elution

Antibody‐antigen binding depends upon ionic, hydrophobic, and hydrogen bonds, as well as van der Waals forces and three‐dimensional conformation. Antibody elution techniques attempt to break those forces by alterations of ionic strength, pH, thermal agitation, and the use of organic solvents. Because of the heterogeneity of the physical forces involved in binding, no single elution technique finds universal applicability to the disruption of all types of antibody‐antigen bonds.