Construction and Characterization of cDNA Library from Peripheral Immune Organ of Merino Sheep

A cDNA library from peripheral immune organs of Merino sheep was constructed to screen genes related to Immunal Reaction and explore their functions.Total RNA were extracted from peripheral immune organs of Merino sheep and mRNA were purified to construct the cDNA library as template,according to the protocol of ZAP Express cDNA Synthesis Kit.Results showed that the cDNA library consisting of a primary contenet of 3.28×105 pfu and on amplified tite of 3.8×108 pfu/mL was constructed with the recombinant ratio of 97.7%.The average inserted fragments were about 1.5 kb with the majority ranging from 0.5 to 2.3 kb.It was concluded that the successfully constructed cDNA library has established a solid molecular basis for further study.