Enzyme-linked immunosorbent assay method for detection of diarrhetic shellfish poison in shellfish along the coast of china

An indirect competitive enzyme-linked immunosorbent assay(idc-ELISA) method for detection of okadaic acid was developed by preparation of the polyclonal antibody against okadaic acid(OA) to investigate the contamination status of shellfish with diarrhetic shellfish poison(DSP) along the coast of China.The content of DSP in shellfish samples was detected and compared with that of the mouse bioassay method.Experimental results show that the detection limit of OA is 0.1 ng/mL,which is equivalent to 8 ng/g for shellfish meat using idc-ELISA.The concordance rate of both the idc-ELISA method and the mouse bioassay method is 90%. DSP concentrations in some shellfish samples are above the regulatory limit of 0.2 μg/g(okadaic acid equivalent).