Surface modification of Nano-hydroxyapatite as carrier of BMP-2 to cell transfection

Objective:To investigate the expression of bone morphagenetic protein 2(BMP-2) gene mediated by hydroxyapatite(HA) nanoparticles with chitosan(CS) into osteoblast and the impact of BMP-2 on osteoblast.Methods:The surface of HA nanoparticles was modified by CS,and the osteoblast was recovered and cultured.The BMP-2 gene was transfected into osteoblast mediated by HA-CS nanoparticles,and RT-PCR was used to identify the expression of BMP-2.The activity of osteoblast was studied by alkaline phoaphatase(ALP) and osteocalcin(OCN).Results:RT-PCR demonstrated the BMP-2 gene was steadily expressed in the osteoblast and enhanced the bone ALP group activity and increased the content of OCN after transfection.Conclusion:HA-CS nanoparticle as a carrier can safely transfect target gene BMP-2 into osteoblast and express steadily by itself and increase the osteoblast biological activity.